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Those who became qualified to receive the initiation of antiretroviral therapy regarding to national guidelines had been actively counseled to initiate treatment and referred to local clinics. End Factors The primary end point was seropositivity in partners previously seronegative for HIV-1. Monthly HIV-1 serologic testing included two rapid HIV-1 antibody assessments in parallel. Study medication was temporarily withheld if either test exposed seroreactivity and was permanently discontinued if enzyme-immunoassay tests confirmed HIV-1 acquisition . Because the study medication was taken by the seronegative partner, HIV-1 sequence analysis to assess transmitting within the analysis partnership was not necessary for end-point determination and was not performed.